Forward genetics screening of Medicago truncatula Tnt1 insertion lines.
Identifieur interne : 000648 ( Main/Exploration ); précédent : 000647; suivant : 000649Forward genetics screening of Medicago truncatula Tnt1 insertion lines.
Auteurs : Juan Carlos Serrani Yarce [États-Unis] ; Hee-Kyung Lee ; Million Tadege ; Pascal Ratet ; Kirankumar S. MysoreSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2013.
Descripteurs français
- KwdFr :
- Medicago truncatula (croissance et développement), Medicago truncatula (génétique), Medicago truncatula (microbiologie), Mutagenèse par insertion (MeSH), Mutation (MeSH), Phénotype (MeSH), Rhizobiaceae (physiologie), Rétroéléments (MeSH), Symbiose (génétique), Tests de criblage à haut débit (méthodes), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Medicago truncatula.
- génétique : Medicago truncatula, Symbiose.
- microbiologie : Medicago truncatula.
- méthodes : Tests de criblage à haut débit.
- physiologie : Rhizobiaceae.
- Mutagenèse par insertion, Mutation, Phénotype, Rétroéléments, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- High-Throughput Screening Assays (methods), Medicago truncatula (genetics), Medicago truncatula (growth & development), Medicago truncatula (microbiology), Mutagenesis, Insertional (MeSH), Mutation (MeSH), Phenotype (MeSH), Plants, Genetically Modified (MeSH), Retroelements (MeSH), Rhizobiaceae (physiology), Symbiosis (genetics).
- MESH :
- chemical : Retroelements.
- genetics : Medicago truncatula, Symbiosis.
- growth & development : Medicago truncatula.
- methods : High-Throughput Screening Assays.
- microbiology : Medicago truncatula.
- physiology : Rhizobiaceae.
- Mutagenesis, Insertional, Mutation, Phenotype, Plants, Genetically Modified.
Abstract
A large population of Medicago truncatula insertion lines has been generated using the Tnt1 retrotransposon. More than 21,000 insertion lines have been generated, representing more than 500,000 insertion events. This mutant population is being used by the legume research community to screen for various different mutants using a forward genetics approach. Some of the phenotypes that have been screened using this population include developmentally abnormal phenotypes in leaves, stem, flowers, and roots. In addition to these, mutants with defects in symbiosis with Rhizobium and mycorrhiza, mutants with altered nonhost resistance against Asian Soybean Rust and switch grass rust pathogens, mutants with altered lignin content, mutants with altered cell wall structure, etc. have been identified. Here, we describe the high throughput methodology that is being used to identify these M. truncatula mutants.
DOI: 10.1007/978-1-62703-613-9_8
PubMed: 23996311
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Mysore</name></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>High-Throughput Screening Assays (methods)</term><term>Medicago truncatula (genetics)</term><term>Medicago truncatula (growth & development)</term><term>Medicago truncatula (microbiology)</term><term>Mutagenesis, Insertional (MeSH)</term><term>Mutation (MeSH)</term><term>Phenotype (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Retroelements (MeSH)</term><term>Rhizobiaceae (physiology)</term><term>Symbiosis (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Medicago truncatula (croissance et développement)</term><term>Medicago truncatula (génétique)</term><term>Medicago truncatula (microbiologie)</term><term>Mutagenèse par insertion (MeSH)</term><term>Mutation (MeSH)</term><term>Phénotype (MeSH)</term><term>Rhizobiaceae (physiologie)</term><term>Rétroéléments (MeSH)</term><term>Symbiose (génétique)</term><term>Tests de criblage à haut débit (méthodes)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Retroelements</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Medicago truncatula</term><term>Symbiosis</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Medicago truncatula</term><term>Symbiose</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>High-Throughput Screening Assays</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Tests de criblage à haut débit</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Rhizobiaceae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Rhizobiaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Mutagenesis, Insertional</term><term>Mutation</term><term>Phenotype</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Mutagenèse par insertion</term><term>Mutation</term><term>Phénotype</term><term>Rétroéléments</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A large population of Medicago truncatula insertion lines has been generated using the Tnt1 retrotransposon. More than 21,000 insertion lines have been generated, representing more than 500,000 insertion events. This mutant population is being used by the legume research community to screen for various different mutants using a forward genetics approach. Some of the phenotypes that have been screened using this population include developmentally abnormal phenotypes in leaves, stem, flowers, and roots. In addition to these, mutants with defects in symbiosis with Rhizobium and mycorrhiza, mutants with altered nonhost resistance against Asian Soybean Rust and switch grass rust pathogens, mutants with altered lignin content, mutants with altered cell wall structure, etc. have been identified. Here, we describe the high throughput methodology that is being used to identify these M. truncatula mutants. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23996311</PMID><DateCompleted><Year>2014</Year><Month>03</Month><Day>26</Day></DateCompleted><DateRevised><Year>2013</Year><Month>09</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1069</Volume><PubDate><Year>2013</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Forward genetics screening of Medicago truncatula Tnt1 insertion lines.</ArticleTitle><Pagination><MedlinePgn>93-100</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-62703-613-9_8</ELocationID><Abstract><AbstractText>A large population of Medicago truncatula insertion lines has been generated using the Tnt1 retrotransposon. More than 21,000 insertion lines have been generated, representing more than 500,000 insertion events. This mutant population is being used by the legume research community to screen for various different mutants using a forward genetics approach. Some of the phenotypes that have been screened using this population include developmentally abnormal phenotypes in leaves, stem, flowers, and roots. In addition to these, mutants with defects in symbiosis with Rhizobium and mycorrhiza, mutants with altered nonhost resistance against Asian Soybean Rust and switch grass rust pathogens, mutants with altered lignin content, mutants with altered cell wall structure, etc. have been identified. Here, we describe the high throughput methodology that is being used to identify these M. truncatula mutants. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yarce</LastName><ForeName>Juan Carlos Serrani</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hee-Kyung</ForeName><Initials>HK</Initials></Author><Author ValidYN="Y"><LastName>Tadege</LastName><ForeName>Million</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Ratet</LastName><ForeName>Pascal</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Mysore</LastName><ForeName>Kirankumar S</ForeName><Initials>KS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018626">Retroelements</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D057166" MajorTopicYN="N">High-Throughput Screening Assays</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046913" MajorTopicYN="N">Medicago truncatula</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016254" MajorTopicYN="Y">Mutagenesis, Insertional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018626" MajorTopicYN="Y">Retroelements</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012230" MajorTopicYN="N">Rhizobiaceae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23996311</ArticleId><ArticleId IdType="doi">10.1007/978-1-62703-613-9_8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oklahoma</li></region></list><tree><noCountry><name sortKey="Lee, Hee Kyung" sort="Lee, Hee Kyung" uniqKey="Lee H" first="Hee-Kyung" last="Lee">Hee-Kyung Lee</name><name sortKey="Mysore, Kirankumar S" sort="Mysore, Kirankumar S" uniqKey="Mysore K" first="Kirankumar S" last="Mysore">Kirankumar S. Mysore</name><name sortKey="Ratet, Pascal" sort="Ratet, Pascal" uniqKey="Ratet P" first="Pascal" last="Ratet">Pascal Ratet</name><name sortKey="Tadege, Million" sort="Tadege, Million" uniqKey="Tadege M" first="Million" last="Tadege">Million Tadege</name></noCountry><country name="États-Unis"><region name="Oklahoma"><name sortKey="Yarce, Juan Carlos Serrani" sort="Yarce, Juan Carlos Serrani" uniqKey="Yarce J" first="Juan Carlos Serrani" last="Yarce">Juan Carlos Serrani Yarce</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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